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The systematic comparison of every newly determined amino acid sequence with all other known sequences may allow a complete reconstruction of the evolutionary events leading to contemporary proteins. But sometimes the surviving similarities are so vague that even computer-based sequence comparison procedures are unable to validate relationships.. Jan 19, 2022 · The third line is the subject sequence (ancient human), and the one below shows the amino acid translation for the subject sequence. Note that there are two additional amino acids, M (methionine) and P (proline), at the beginning of the protein sequence in modern humans compared to Neanderthal..

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Segment methods compare all windows (overlapping segments of a predetermined length (e.g., 10 amino acids)) from one sequence to all segments from the other. This is the approach used in dotplots. Optimal global alignment methods allow the best overall score for the comparison of the two sequences to be obtained, including a consideration of gaps.

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